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Argylla DNA nanoPurify Kit - A10

Argylla DNa nanoPurify KitBased on Nanochromatography, this kit is for purifying and further concentrating DNA from samples with as little as 100 picograms up to 15 micrograms of DNA. This approach avoids the need for carrier DNA, RNA, glycogen or other coprecipitants that might compromise downstream molecular biology applications. The primary applications of this kit are:

  1. To process DNA from samples lysed by other front-end processes.
  2. To process DNA from reactions such as PCR, whole genome amplification (WGA), enzyme digests or labeling reactions,
  3. To further purify and concentrate DNA extracted by methods based on spin columns, magnetic beads, microfiltration, protease-digests or organic solvent extraction,
  4. To further concentrate dilute DNA eluates isolated at dilute concentrations by any of these means

The process ensures very high input to recovery ratios and eluted DNA demonstrates the integrity to support enzyme labeling, PCR, WGA, Southern blotting and other hybridization assays.

Code Kit sizes   Price
A10M011 Mini 50 standard 50µL samples, scales from 25 to 100 samples   60.00
A10M013 Standard 250 standard 50µL samples, scales from 125 to 500 samples 200.00

Application Examples - nanoPurify Kit

Purification of Oligonucleotides - When "Standard Desalting" is not enoughOligonucleotides

Argylla nanoPurify Kit cleans otherwise trusty PCR primers for a slightly cleaner PCR product. Clean primers are more efficient per unit mass since they are depleated of UV-absorping material contributing to the apparant oligo mass as determined by UV. Also, heavily contaminated primers sometimes show "shadow bands" in the PCR product mix as seen on a native gel, due to inapproproate PCR initiation. What you will obtain upon cleanup is more PCR product, and less shorter, inappropriately initiated products. Read more..

Remove Flourescent Dye from Lonza© Recovery Well DNA.

The Lonza© recovery gel is an excellent product for purifying small pieces of DNA. HOWEVER, there is concern that the fluorescent tracking dye might interfere with DNA quantification using 260 nm absorbance as measured by the NanoDrop spectrophotometer. Argylla's nanoPurify Kit separates the Lonza fluorescent tracking dye from the DNA removed from the Lonza recovery gels.

The fluorescent DNA tracking probe does not interfere with traditional PCR, but the dye may interfere with quantifying DNA concentration either by PicoGreen or by UV absorbance as measured by the NanoDrop spectrophotometer. Testing has determined our ability to remove the fluorescent dye from two different sizes of PCR amplified DNA isolated from the Lonza recovery wells. Read more...

Concentrate and Desalt DNA Purified by Other Technologies or the DNA Products from Nucleic Acid Amplification.

The DNA nanoPurify Kit with PrepParticles is readily optimized to purify, concentrate and desalt the DNA which has been purified by phenol extraction or mini-column purification or the reaction products resulting from whole genome amplification (WGA), PCR or microarray labeling reactions. PrepParticle based concentration has proven to be particularly useful when the DNA is in the 1-100ng scale and must be recovered, without loss, into a final volume of less than 50 microliters. That final concentration factor can be as much as 50-fold and final DNA concentrations can be as high as 100 ng/µL. For most samples, overall recovery is greater than 80%. DNA which has been concentrated and de-salted by this protocol have been shown to support SNP analysis via TaqMan™ (ABI) and Luminex™ bead arrays. Validation via the use of Affymetrix™ 500 K SNP Chip are in progress. top

Swabs

Purify and Concentrate DNA from Buccal Swabs

DNA collection via a cheek wipe has become the standard in large-scale Buccal swabs forensic database development and, more recently, for population-scale genetic epidemiology. The literature suggests that DNA yields from such cheek (buccal) wipes are in the 0.2 to 1 microgram range. Argylla has developed a PrepParticle protocol for the quick and easy purification and concentration of DNA obtained from such cheek wipes. Briefly, cells are collected by wiping the inner cheek with a sterile cotton swab followed by air-drying. The swab is then re-hydrated and subjected to protease digestion by the FBI-standard process for 4 hours at 56C, followed by centrifugation of the swab to isolate the fluid sample in about ½ ml. The raw, protease digested DNA sample is then diluted to 500 microliters, followed by a standard PrepParticle isolation or by Argylla's optimized extraction process. The resulting DNA preparation can then be analyzed by the standard human Identifiler STR panel or any other PCR based test. Quantitation via PicoGreen fluorescence demonstrates DNA yields in the 500ng to 2µg range. Because the sample is purified, concentrated and released for analysis as a one-pot reaction, sample tracking and chain of custody are greatly enhanced.
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